Are undetermined "n" nucleotides in the V-REGION counted as mutated nucleotides in IMGT/V-QUEST analysis?
In IMGT/V-QUEST analysis, undetermined "n" nucleotides in the V-REGION are counted as mutated nucleotides.
Their number is explicitly indicated in the results as "V-REGION uncertain nt nb":
- below the Result summary in 'A. Detailed view' display,
- in a column in 'B. Synthesis view' display,
- in 'nt-sequences' in 'C. Excel file' (equivalent to 'nt-sequences' CSV file of IMGT/HighV-QUEST results)