Antibody engineering

IMGT Primer-DB

IMGT Sequence databases

IMGT Sequence analysis tools

IMGT Genome database

IMGT Genome analysis tools

IMGT 3D structure database

IMGT Structure analysis tools

IMGT Web resources

IMGT links

Other links

Antibody engineering

Combinatorial libraries & phage displays

Reagents monoclonal antibodies

Antibody glycosylation and effector properties

Antibody humanization

Alemtuzumab CAMPATH-1H (Humanized antibodies)

K20 (Humanized antibodies)

  • Protein display V-DOMAIN:
  • Colliers de Perles:

FR-IMGT and CDR-IMGT

Antibody camelization

Bispecific antibodies

Antibody drug conjugates (ADC)

Transgenic animals for human antibody production

  • Transgenic animals created for the production of human antibodies include :
    • Medarex�s UltiMAb Human Antibody Development System� transgenic mice [1]
    • Minilocus transgenic mice [2]
    • Abgenix's XenoMouse� transgenic mouse [3]
    • Double trans-chromosomic mice [4]
    • TransChromo Mouse [5]
    • VelocImmune� mice technology [6]
They are reported, if known, in IMGT/mAb-DB, in �Development technology�. For a review and future developments, see [7].

Monoclonal antibodies with clinical indications

Fusion proteins for immune applications (FPIA)

External links and resources

References:
[1] Lonberg N et al. Antigen-specific human antibodies from mice comprising four distinct genetic modifications. Nature 368(6474):856-859 (1994).PMID: 8159246
[2] Fishwild DM et al. High-avidity human IgG kappa monoclonal antibodies from a novel strain of minilocus transgenic mice. Nat Biotechnol. 14(7):845-851 (1996). PMID: 9631008 PMID: 9631008
[3] Mendez MJ et al. Functional transplant of megabase human immunoglobulin loci recapitulates human antibody response in mice. Nat Genet. 15(2):146-156 (1997). Erratum in: Nat Genet. 16(4):410 (1997). PMID: 9020839
[4] Tomizuka K et al. Double trans-chromosomic mice: maintenance of two individual human chromosome fragments containing Ig heavy and kappa loci and expression of fully human antibodies. Proc Natl Acad Sci U S A. 97(2):722-727 (2000). PMID: 10639146
[5] Ishida I et al. Production of human monoclonal and polyclonal antibodies in TransChromo animals. Cloning Stem Cells 4(1):91-102 (2002). PMID: 12006160
[6] Murphy AJ et al. Mice with megabase humanization of their immunoglobulin genes generate antibodies as efficiently as normal mice. Proc Natl Acad Sci U S A. 111(14):5153-5158 (2014). doi: 10.1073/pnas.1324022111. Epub 2014 Mar 25. PMID: 24706856
[7] Br�ggemann M et al. Human antibody production in transgenic animals. Arch. Immunol. Ther. Exp. (Warsz) 63(2):101-108 (2015). doi: 10.1007/s00005-014-0322-x. Epub 2014 Dec 3. Review. PMID: 2546794