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Database contains
247103 sequences
from 361 species

IMGT/LIGM-DB sequence

Version:
1.2.9
Database release:
LIGMDB_V12
Citing IMGT/LIGM-DB:
Giudicelli, V., Duroux P., Ginestoux C., Folch G., Jabado-Michaloud J., Chaume D., Lefranc M.-P., Nucleic Acids Res., 34:D781-D784 (2006). PMID:16381979 Abstract Full PDF

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AF103571 - Annotation

Sections
Accession number AF103571
Sequence version 1
Secondary accession numbers
IMGT annotation level by annotators
Definition Homo sapiens isolate donor Z clone Z4K immunoglobulin kappa light chain variable region mRNA, partial cds.
Species Homo sapiens (human)
Taxonomy cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Primates; Haplorrhini; Simiiformes; Catarrhini; Hominoidea; Hominidae; Homininae; Homo; Homo sapiens
Sequence length 330
IMGT/LIGM-DB dates 26-JUN-2000
  15-JAN-2013 (v. 11)

OBTENTION

Specificities
Disease related

IDENTIFICATION

Molecule type cDNA
Configuration type undefined, rearranged
Gene type constant, variable, joining
Molecule entity type L-V-J-C-sequence
Functionality type productive
Chain type IG-Light, IG-Light-Kappa-IGK
Structure type regular
Location type
Other keywords antigen receptor, Immunoglobulin superfamily (IgSF), immunoglobulin (IG), IMGT reference sequence

CLASSIFICATION

IMGT Groups IGKV
IMGT Subgroups IGKV3

DESCRIPTION

L-V-J-C-SEQUENCE   <1..>330
  cell_type B cell
  clone Z4K
  db_xref taxon:9606
  FR_length [20.17.36.10]
  isolate donor Z
  note isolated from patient with systemic lupus erythematosus
  organism Homo sapiens
  protein_id AAD16631.1
  tissue_type peripheral blood
V-J-C-REGION   <1..>330
tctccagccaccctgtctgtgtctccaggggaaagagccaccctctcctgcagggccagtcagagtgttagcggccacttagcctggtaccagcagaaacctggccaggctcccaggctcctcatctatgctggatccaccagggccactggtgtcccagccaggttcagtggcagtgggtctgggacagatttcactctcagcatcagcagcctgcagtctgaagattgtgcagtttattactgtcaacagtacaataactggtctccgctcacgttcggcggagggaccaaggtggagatcaaacgaactgggctgcacctcttaatg S P A T L S V S P G E R A T L S C R A S Q S V S G H L A W Y Q Q K P G Q A P R L L I Y A G S T R A T G V P A R F S G S G S G T D F T L S I S S L Q S E D C A V Y Y C Q Q Y N N W S P L T F G G G T K V E I K R T G L H L L M
L Q P P C L C L Q G K E P P S P A G P V R V L A A T * P G T S R N L A R L P G S S S M L D P P G P L V S Q P G S V A V G L G Q I S L S A S A A C S L K I V Q F I T V N S T I T G L R S R S A E G P R W R S N E L G C T S *
S S H P V C V S R G K S H P L L Q G Q S E C * R P L S L V P A E T W P G S Q A P H L C W I H Q G H W C P S Q V Q W Q W V W D R F H S Q H Q Q P A V * R L C S L L L S T V Q * L V S A H V R R R D Q G G D Q T N W A A P L N
V-J-REGION   <1..306
tctccagccaccctgtctgtgtctccaggggaaagagccaccctctcctgcagggccagtcagagtgttagcggccacttagcctggtaccagcagaaacctggccaggctcccaggctcctcatctatgctggatccaccagggccactggtgtcccagccaggttcagtggcagtgggtctgggacagatttcactctcagcatcagcagcctgcagtctgaagattgtgcagtttattactgtcaacagtacaataactggtctccgctcacgttcggcggagggaccaaggtggagatcaaa S P A T L S V S P G E R A T L S C R A S Q S V S G H L A W Y Q Q K P G Q A P R L L I Y A G S T R A T G V P A R F S G S G S G T D F T L S I S S L Q S E D C A V Y Y C Q Q Y N N W S P L T F G G G T K V E I K
L Q P P C L C L Q G K E P P S P A G P V R V L A A T * P G T S R N L A R L P G S S S M L D P P G P L V S Q P G S V A V G L G Q I S L S A S A A C S L K I V Q F I T V N S T I T G L R S R S A E G P R W R S
S S H P V C V S R G K S H P L L Q G Q S E C * R P L S L V P A E T W P G S Q A P H L C W I H Q G H W C P S Q V Q W Q W V W D R F H S Q H Q Q P A V * R L C S L L L S T V Q * L V S A H V R R R D Q G G D Q
V-REGION   <1..269
  CDR_length [6.3.10]
  IMGT_allele IGKV3-15*01
  IMGT_gene IGKV3-15
tctccagccaccctgtctgtgtctccaggggaaagagccaccctctcctgcagggccagtcagagtgttagcggccacttagcctggtaccagcagaaacctggccaggctcccaggctcctcatctatgctggatccaccagggccactggtgtcccagccaggttcagtggcagtgggtctgggacagatttcactctcagcatcagcagcctgcagtctgaagattgtgcagtttattactgtcaacagtacaataactggtctcc S P A T L S V S P G E R A T L S C R A S Q S V S G H L A W Y Q Q K P G Q A P R L L I Y A G S T R A T G V P A R F S G S G S G T D F T L S I S S L Q S E D C A V Y Y C Q Q Y N N W S
L Q P P C L C L Q G K E P P S P A G P V R V L A A T * P G T S R N L A R L P G S S S M L D P P G P L V S Q P G S V A V G L G Q I S L S A S A A C S L K I V Q F I T V N S T I T G L
S S H P V C V S R G K S H P L L Q G Q S E C * R P L S L V P A E T W P G S Q A P H L C W I H Q G H W C P S Q V Q W Q W V W D R F H S Q H Q Q P A V * R L C S L L L S T V Q * L V S
FR1-IMGT   <1..60
  AA_IMGT AA 7 to 26
tctccagccaccctgtctgtgtctccaggggaaagagccaccctctcctgcagggccagt S P A T L S V S P G E R A T L S C R A S
L Q P P C L C L Q G K E P P S P A G P
S S H P V C V S R G K S H P L L Q G Q
1st-CYS   49..51
CDR1-IMGT   61..78
  AA_IMGT AA 27 to 38, AA 30, 31, 32, 33, 34 and 35 are missing
cagagtgttagcggccac Q S V S G H
R V L A A
E C * R P
FR2-IMGT   79..129
  AA_IMGT AA 39 to 55
ttagcctggtaccagcagaaacctggccaggctcccaggctcctcatctat L A W Y Q Q K P G Q A P R L L I Y
* P G T S R N L A R L P G S S S
S L V P A E T W P G S Q A P H L
CONSERVED-TRP   85..87
CDR2-IMGT   130..138
  AA_IMGT AA 56 to 65, AA 58, 59, 60, 61, 62, 63 and 64 are missing
gctggatcc A G S
L D
W I
FR3-IMGT   139..246
  AA_IMGT AA 66 to 104, AA 73, 81, 82 are missing
accagggccactggtgtcccagccaggttcagtggcagtgggtctgggacagatttcactctcagcatcagcagcctgcagtctgaagattgtgcagtttattactgt T R A T G V P A R F S G S G S G T D F T L S I S S L Q S E D C A V Y Y C
P G P L V S Q P G S V A V G L G Q I S L S A S A A C S L K I V Q F I T
Q G H W C P S Q V Q W Q W V W D R F H S Q H Q Q P A V * R L C S L L L
2nd-CYS   244..246
CDR3-IMGT   247..276
  AA_IMGT AA 105 to 117, AA 110, 111 and 112 are missing
caacagtacaataactggtctccgctcacg Q Q Y N N W S P L T
N S T I T G L R S
T V Q * L V S A H
JUNCTION   244..279
  in_frame
tgtcaacagtacaataactggtctccgctcacgttc C Q Q Y N N W S P L T F
V N S T I T G L R S R
S T V Q * L V S A H V
J-REGION   270..306
  IMGT_allele IGKJ4*02
  IMGT_gene IGKJ4
gctcacgttcggcggagggaccaaggtggagatcaaa A H V R R R D Q G G D Q
L T F G G G T K V E I K
S R S A E G P R W R S
J-PHE   277..279
C-REGION   307..>330
cgaactgggctgcacctcttaatg R T G L H L L M
E L G C T S *
N W A A P L N

Literature references

[1] 1-330
de Wildt R.M.T., Hoet R.M.A., van Venrooij W.J., Tomlinson I.M., Winter G.
Analysis of heavy and light chain pairings indicates that receptor editing shapes the human antibody repertoire
J. Mol. Biol. 285(3):895-901 (1999)
DOI: 10.1006/jmbi.1998.2396
PUBMED: 9887257
[2] 1-330
de Wildt R.M.T., Hoet R.M.A., van Venrooij W.J., Tomlinson I.M., Winter G.
Submitted to the ENA/GenBank/DDBJ databases (29-OCT-1998)
Centre for Protein Engineering, MRC, Hills Road, Cambridge CB2 2QH, UK

Database cross-references

Database Identifiers Relation
ENA AF103571

Sequence

Length : 330 BP
Composition : 71 A; 98 C; 89 G; 72 T; 0 other

     tctccagcca ccctgtctgt gtctccaggg gaaagagcca ccctctcctg cagggccagt        60
     cagagtgtta gcggccactt agcctggtac cagcagaaac ctggccaggc tcccaggctc       120
     ctcatctatg ctggatccac cagggccact ggtgtcccag ccaggttcag tggcagtggg       180
     tctgggacag atttcactct cagcatcagc agcctgcagt ctgaagattg tgcagtttat       240
     tactgtcaac agtacaataa ctggtctccg ctcacgttcg gcggagggac caaggtggag       300
     atcaaacgaa ctgggctgca cctcttaatg                                        330
Created:
23/07/1995
Database development:
Denys CHAUME, Patrice DUROUX, Nelly JOUFFRE
Database scientific officer:
Marie-Paule Lefranc