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Database contains
247103 sequences
from 361 species

IMGT/LIGM-DB sequence

Version:
1.2.9
Database release:
LIGMDB_V12
Citing IMGT/LIGM-DB:
Giudicelli, V., Duroux P., Ginestoux C., Folch G., Jabado-Michaloud J., Chaume D., Lefranc M.-P., Nucleic Acids Res., 34:D781-D784 (2006). PMID:16381979 Abstract Full PDF

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X63370 - Annotation

Sections
Accession number X63370
Sequence version 1
Secondary accession numbers S37650
IMGT annotation level by annotators
Definition H.sapiens gene for immunoglobulin kappa light chain variable region 'A30'
Species Homo sapiens (human)
Taxonomy cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Primates; Haplorrhini; Simiiformes; Catarrhini; Hominoidea; Hominidae; Homininae; Homo; Homo sapiens
Sequence length 1528
IMGT/LIGM-DB dates 15-MAY-1995
  05-MAR-2013 (v. 22)

OBTENTION

Specificities
Disease related

IDENTIFICATION

Molecule type gDNA
Configuration type germline, rearranged
Gene type variable, joining
Molecule entity type J-gene, V-J-gene
Functionality type pseudogene
Chain type IG-Light, IG-Light-Kappa-IGK
Structure type regular
Location type
Other keywords antigen receptor, Immunoglobulin superfamily (IgSF), immunoglobulin (IG)

CLASSIFICATION

IMGT Groups IGKV
IMGT Subgroups

DESCRIPTION

(VJ)-J-CLUSTER   <1..1528
  cell_line JI
  chromosome 2
  clone JI1, JI2
  db_xref taxon:9606
  IMGT_map 2p11.2
  isolate JI
  mol_type genomic DNA
  organism Homo sapiens
V-J-GENE   <1..636
  note The V-REGION does not correspond to V-GENE IGKV1-17(A30) as said in the definition.
L-PART1   <1..18
tgctgctctggttcccag C C S G S Q
A A L V P
L L W F P
DONOR-SPLICE   18..20
V-INTRON   19..143
ACCEPTOR-SPLICE   141..145
V-J-EXON   144..475
gtgccaggtgtgacatccagatgacccagtctccatcctccctgtctgcatctgtaagacagagtcaccatcacttgccgggcaagtcagggcattagaaatgatttaggctggtatcagcagaaaccagggaaagcccctaagcgcctgatctatgctgcatccagtttgcaaagtggggtcccatcaaggttcagcggcagtggatctgggacagaattcactctcacaatcagcagcctgcagcctgaagattttgcaacttattactgtctacagcataatagttaccctcgggacgttcggccaagggaccaaggtggaaatcaaac V P G V T S R * P S L H P P C L H L * D R V T I T C R A S Q G I R N D L G W Y Q Q K P G K A P K R L I Y A A S S L Q S G V P S R F S G S G S G T E F T L T I S S L Q P E D F A T Y Y C L Q H N S Y P R D V R P R D Q G G N Q
C Q V * H P D D P V S I L P V C I C K T E S P S L A G Q V R A L E M I * A G I S R N Q G K P L S A * S M L H P V C K V G S H Q G S A A V D L G Q N S L S Q S A A C S L K I L Q L I T V Y S I I V T L G T F G Q G T K V E I K
A R C D I Q M T Q S P S S L S A S V R Q S H H H L P G K S G H * K * F R L V S A E T R E S P * A P D L C C I Q F A K W G P I K V Q R Q W I W D R I H S H N Q Q P A A * R F C N L L L S T A * * L P S G R S A K G P R W K S N
V-EXON   144..439
gtgccaggtgtgacatccagatgacccagtctccatcctccctgtctgcatctgtaagacagagtcaccatcacttgccgggcaagtcagggcattagaaatgatttaggctggtatcagcagaaaccagggaaagcccctaagcgcctgatctatgctgcatccagtttgcaaagtggggtcccatcaaggttcagcggcagtggatctgggacagaattcactctcacaatcagcagcctgcagcctgaagattttgcaacttattactgtctacagcataatagttaccctcg V P G V T S R * P S L H P P C L H L * D R V T I T C R A S Q G I R N D L G W Y Q Q K P G K A P K R L I Y A A S S L Q S G V P S R F S G S G S G T E F T L T I S S L Q P E D F A T Y Y C L Q H N S Y P
C Q V * H P D D P V S I L P V C I C K T E S P S L A G Q V R A L E M I * A G I S R N Q G K P L S A * S M L H P V C K V G S H Q G S A A V D L G Q N S L S Q S A A C S L K I L Q L I T V Y S I I V T L
A R C D I Q M T Q S P S S L S A S V R Q S H H H L P G K S G H * K * F R L V S A E T R E S P * A P D L C C I Q F A K W G P I K V Q R Q W I W D R I H S H N Q Q P A A * R F C N L L L S T A * * L P S
L-PART2   144..154
gtgccaggtgt V P G
C Q V
A R C
V-J-REGION   155..475
gacatccagatgacccagtctccatcctccctgtctgcatctgtaagacagagtcaccatcacttgccgggcaagtcagggcattagaaatgatttaggctggtatcagcagaaaccagggaaagcccctaagcgcctgatctatgctgcatccagtttgcaaagtggggtcccatcaaggttcagcggcagtggatctgggacagaattcactctcacaatcagcagcctgcagcctgaagattttgcaacttattactgtctacagcataatagttaccctcgggacgttcggccaagggaccaaggtggaaatcaaac D I Q M T Q S P S S L S A S V R Q S H H H L P G K S G H * K * F R L V S A E T R E S P * A P D L C C I Q F A K W G P I K V Q R Q W I W D R I H S H N Q Q P A A * R F C N L L L S T A * * L P S G R S A K G P R W K S N
T S R * P S L H P P C L H L * D R V T I T C R A S Q G I R N D L G W Y Q Q K P G K A P K R L I Y A A S S L Q S G V P S R F S G S G S G T E F T L T I S S L Q P E D F A T Y Y C L Q H N S Y P R D V R P R D Q G G N Q
H P D D P V S I L P V C I C K T E S P S L A G Q V R A L E M I * A G I S R N Q G K P L S A * S M L H P V C K V G S H Q G S A A V D L G Q N S L S Q S A A C S L K I L Q L I T V Y S I I V T L G T F G Q G T K V E I K
V-REGION   155..439
  pseudo Because of a frameshift in FR1
gacatccagatgacccagtctccatcctccctgtctgcatctgtaagacagagtcaccatcacttgccgggcaagtcagggcattagaaatgatttaggctggtatcagcagaaaccagggaaagcccctaagcgcctgatctatgctgcatccagtttgcaaagtggggtcccatcaaggttcagcggcagtggatctgggacagaattcactctcacaatcagcagcctgcagcctgaagattttgcaacttattactgtctacagcataatagttaccctcg D I Q M T Q S P S S L S A S V R Q S H H H L P G K S G H * K * F R L V S A E T R E S P * A P D L C C I Q F A K W G P I K V Q R Q W I W D R I H S H N Q Q P A A * R F C N L L L S T A * * L P S
T S R * P S L H P P C L H L * D R V T I T C R A S Q G I R N D L G W Y Q Q K P G K A P K R L I Y A A S S L Q S G V P S R F S G S G S G T E F T L T I S S L Q P E D F A T Y Y C L Q H N S Y P
H P D D P V S I L P V C I C K T E S P S L A G Q V R A L E M I * A G I S R N Q G K P L S A * S M L H P V C K V G S H Q G S A A V D L G Q N S L S Q S A A C S L K I L Q L I T V Y S I I V T L
FR1   155..221
  note Frameshift
gacatccagatgacccagtctccatcctccctgtctgcatctgtaagacagagtcaccatcacttgc D I Q M T Q S P S S L S A S V R Q S H H H L
T S R * P S L H P P C L H L * D R V T I T C
H P D D P V S I L P V C I C K T E S P S L
CDR1   222..254
cgggcaagtcagggcattagaaatgatttaggc R A S Q G I R N D L G
G Q V R A L E M I *
G K S G H * K * F R
FR2   255..299
tggtatcagcagaaaccagggaaagcccctaagcgcctgatctat W Y Q Q K P G K A P K R L I Y
G I S R N Q G K P L S A * S
V S A E T R E S P * A P D L
CDR2   300..320
gctgcatccagtttgcaaagt A A S S L Q S
L H P V C K
C I Q F A K
FR3   321..416
ggggtcccatcaaggttcagcggcagtggatctgggacagaattcactctcacaatcagcagcctgcagcctgaagattttgcaacttattactgt G V P S R F S G S G S G T E F T L T I S S L Q P E D F A T Y Y C
G S H Q G S A A V D L G Q N S L S Q S A A C S L K I L Q L I T
G P I K V Q R Q W I W D R I H S H N Q Q P A A * R F C N L L L
CDR3   417..444
ctacagcataatagttaccctcgggacg L Q H N S Y P R D
Y S I I V T L G T
T A * * L P S G
JUNCTION   414..447
  out_of_frame
tgtctacagcataatagttaccctcgggacgttc C L Q H N S Y P R D V
V Y S I I V T L G T F
S T A * * L P S G R
J-REGION   440..475
  IMGT_allele IGKJ1*01
  IMGT_gene IGKJ1
ggacgttcggccaagggaccaaggtggaaatcaaac G R S A K G P R W K S N
D V R P R D Q G G N Q
T F G Q G T K V E I K
DONOR-SPLICE   475..477
3'UTR   476..636
J-CLUSTER   637..1528
J-GENE   637..951
  IMGT_allele IGKJ2*01
  IMGT_gene IGKJ2
5'UTR   637..797
J-NONAMER   760..768
J-SPACER   769..790
J-HEPTAMER   791..797
J-REGION   798..836
  IMGT_allele IGKJ2*01
  IMGT_gene IGKJ2
tgtacacttttggccaggggaccaagctggagatcaaac C T L L A R G P S W R S N
V H F W P G D Q A G D Q
Y T F G Q G T K L E I K
J-PHE   806..808
DONOR-SPLICE   836..838
3'UTR   837..951
J-GENE   952..1528
  IMGT_allele IGKJ3*01
  IMGT_gene IGKJ3
5'UTR   952..1002
J-NONAMER   1066..1074
J-SPACER   1075..1095
J-HEPTAMER   1096..1102
J-REGION   1103..1140
  IMGT_allele IGKJ3*01
  IMGT_gene IGKJ3
attcactttcggccctgggaccaaagtggatatcaaac I H F R P W D Q S G Y Q
F T F G P G T K V D I K
S L S A L G P K W I S N
J-PHE   1110..1112
DONOR-SPLICE   1140..1142
3'UTR   1141..1528

Literature references

[1] 1-1528
Huber C.F.M.
Submitted to the ENA/GenBank/DDBJ databases (27-NOV-1991)
C.F.M. Huber, Inst f Physiol Chemie, Uni Muenchen, Schillerstrasse 44, D-8000 Muenchen 2, FRG
[2] 1-1528
Huber C., Klobeck H.G., Zachau H.G.
Ongoing V kappa-J kappa recombination after formation of a productive V kappa-J kappa coding joint
Eur. J. Immunol. 22(6):1561-1565 (1992)
PUBMED: 1601042

Database cross-references

Database Identifiers Relation
ENA X63370

Sequence

Length : 1528 BP
Composition : 420 A; 303 C; 369 G; 436 T; 0 other

     tgctgctctg gttcccaggt aaggatggag aacactagca gtttactcag cccagagtgc        60
     tcagtactgc tttactgttc agggaaattc tcttacaaca tgattaattg tgtggacatt       120
     tgtttttatg tttccaatct caggtgccag gtgtgacatc cagatgaccc agtctccatc       180
     ctccctgtct gcatctgtaa gacagagtca ccatcacttg ccgggcaagt cagggcatta       240
     gaaatgattt aggctggtat cagcagaaac cagggaaagc ccctaagcgc ctgatctatg       300
     ctgcatccag tttgcaaagt ggggtcccat caaggttcag cggcagtgga tctgggacag       360
     aattcactct cacaatcagc agcctgcagc ctgaagattt tgcaacttat tactgtctac       420
     agcataatag ttaccctcgg gacgttcggc caagggacca aggtggaaat caaacgtgag       480
     tagaatttaa actttgcttc ctcagttgtc tgtgtcttct gttccctgtg tctatgaagt       540
     gatctataag gtgactctgc aatcagcctc tgatatcctt cagggaaaag ataaagataa       600
     gtctgtagtc aaactcgaga attgattgca cattttcttt gaagagcaag caagattcag       660
     tcattgggtg agaataactt gtctaagtaa tagcttcaga aatgtcctgg ggaacataac       720
     atgttctgga cagagccttg gtcaattgtc agaaagggag tttttgtata ggagggaagt       780
     taagaggaac cattgtgtgt acacttttgg ccaggggacc aagctggaga tcaaacgtaa       840
     gtactttttt ccactgattc ttcactgttg ctaattagtt tactttgtgt tcctttgtgt       900
     ggattttcat tagtcggatg ccagggatct aacaaacttc attcccaggt taggtacaga       960
     ggaggggaaa ttgttccaca ggacgctagc ttgtggctaa tttttaagat ttctaaatca      1020
     aaataacttc attgggggaa agaggcttgc tgagctttca gggaggtttt tgtaaaggga      1080
     aaagttaaga cgaatcactg tgattcactt tcggccctgg gaccaaagtg gatatcaaac      1140
     gtaagtacat ctgtctcaat tattcgtgag attttagtgc cattgtatca tttgtgcaag      1200
     ttttgtgata ttttggttga ataaacctgg tgacccagaa gtaaatagca ggacaccaga      1260
     aaatgaactt aaaaagctga gcaaatagac gaatcattgg gtttgagagg agaataggat      1320
     tcatggggga aatggggaag aaatagctag atttttctct gaacaagcag cctatctcat      1380
     atgattggct ccaagagagg tttttgttga ggggaaaggg tgagatccct cactgtgcac      1440
     agtgcttcag cctcgaacac aaacctcctc cccatacgct gggccagtag gtctttgctg      1500
     cagcagctgc ttcctctgca cacagccc                                         1528
Created:
23/07/1995
Database development:
Denys CHAUME, Patrice DUROUX, Nelly JOUFFRE
Database scientific officer:
Marie-Paule Lefranc