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Database contains
247103 sequences
from 361 species

IMGT/LIGM-DB sequence

Version:
1.2.9
Database release:
LIGMDB_V12
Citing IMGT/LIGM-DB:
Giudicelli, V., Duroux P., Ginestoux C., Folch G., Jabado-Michaloud J., Chaume D., Lefranc M.-P., Nucleic Acids Res., 34:D781-D784 (2006). PMID:16381979 Abstract Full PDF

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Z46620 - Annotation

Sections
Accession number Z46620
Sequence version 1
Secondary accession numbers
IMGT annotation level automatic
Definition H.sapiens mRNA for immunoglobulin kappa chain (g21).
Species Homo sapiens (human)
Taxonomy cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Primates; Haplorrhini; Simiiformes; Catarrhini; Hominoidea; Hominidae; Homininae; Homo; Homo sapiens
Sequence length 342
IMGT/LIGM-DB dates 15-MAY-1995
  06-DEC-2012 (v. 12)

OBTENTION

Specificities
Disease related

IDENTIFICATION

Molecule type cDNA
Configuration type undefined, rearranged
Gene type variable, joining
Molecule entity type L-V-J-C-sequence
Functionality type productive
Chain type IG-Light, IG-Light-Kappa-IGK
Structure type regular
Location type
Other keywords antigen receptor, Immunoglobulin superfamily (IgSF), immunoglobulin (IG), IMGT reference sequence

CLASSIFICATION

IMGT Groups IGKV
IMGT Subgroups IGKV2

DESCRIPTION

L-V-J-C-SEQUENCE   <1..>342
  cell_type B-lymphocyte
  clone G21
  IMGT_note automatically annotated with IMGT tools
  organism Homo sapiens
  productive
  tissue_type blood
V-J-C-REGION   1..>342
  citation [1]
  product immunoglobulin kappa chain precursor
gatattgtgatgactcagtctccactctccctgcccgtcacccctggagagccggcctccatctcctgcaggtctagtcagagcctcctgcatagtaatggatacaactatttggattggtacctgcagaagccagggcagtctccacagctcctgatctatttgggttctaatcgggcctccggggtccctgacaggttcagtggcagtggatcaggcacagattttacactgaaaatcagcagagtggaggctgaggatgttggggtttattactgcatgcaagctctacaaactctcatgtgcagttttggccaggggaccaagctggagatcaaacga D I V M T Q S P L S L P V T P G E P A S I S C R S S Q S L L H S N G Y N Y L D W Y L Q K P G Q S P Q L L I Y L G S N R A S G V P D R F S G S G S G T D F T L K I S R V E A E D V G V Y Y C M Q A L Q T L M C S F G Q G T K L E I K R
I L * * L S L H S P C P S P L E S R P P S P A G L V R A S C I V M D T T I W I G T C R S Q G S L H S S * S I W V L I G P P G S L T G S V A V D Q A Q I L H * K S A E W R L R M L G F I T A C K L Y K L S C A V L A R G P S W R S N
Y C D D S V S T L P A R H P W R A G L H L L Q V * S E P P A * * W I Q L F G L V P A E A R A V S T A P D L F G F * S G L R G P * Q V Q W Q W I R H R F Y T E N Q Q S G G * G C W G L L L H A S S T N S H V Q F W P G D Q A G D Q T
V-J-REGION   1..339
  CDR_length [11.3.10]
  FR_length [26.17.36.10]
gatattgtgatgactcagtctccactctccctgcccgtcacccctggagagccggcctccatctcctgcaggtctagtcagagcctcctgcatagtaatggatacaactatttggattggtacctgcagaagccagggcagtctccacagctcctgatctatttgggttctaatcgggcctccggggtccctgacaggttcagtggcagtggatcaggcacagattttacactgaaaatcagcagagtggaggctgaggatgttggggtttattactgcatgcaagctctacaaactctcatgtgcagttttggccaggggaccaagctggagatcaaa D I V M T Q S P L S L P V T P G E P A S I S C R S S Q S L L H S N G Y N Y L D W Y L Q K P G Q S P Q L L I Y L G S N R A S G V P D R F S G S G S G T D F T L K I S R V E A E D V G V Y Y C M Q A L Q T L M C S F G Q G T K L E I K
I L * * L S L H S P C P S P L E S R P P S P A G L V R A S C I V M D T T I W I G T C R S Q G S L H S S * S I W V L I G P P G S L T G S V A V D Q A Q I L H * K S A E W R L R M L G F I T A C K L Y K L S C A V L A R G P S W R S
Y C D D S V S T L P A R H P W R A G L H L L Q V * S E P P A * * W I Q L F G L V P A E A R A V S T A P D L F G F * S G L R G P * Q V Q W Q W I R H R F Y T E N Q Q S G G * G C W G L L L H A S S T N S H V Q F W P G D Q A G D Q
V-REGION   1..298
  IMGT_allele IGKV2-28*01 or IGKV2D-28*01
  IMGT_gene IGKV2-28 or IGKV2D-28
  Videntity 100,00% (298/298 nt)
gatattgtgatgactcagtctccactctccctgcccgtcacccctggagagccggcctccatctcctgcaggtctagtcagagcctcctgcatagtaatggatacaactatttggattggtacctgcagaagccagggcagtctccacagctcctgatctatttgggttctaatcgggcctccggggtccctgacaggttcagtggcagtggatcaggcacagattttacactgaaaatcagcagagtggaggctgaggatgttggggtttattactgcatgcaagctctacaaactc D I V M T Q S P L S L P V T P G E P A S I S C R S S Q S L L H S N G Y N Y L D W Y L Q K P G Q S P Q L L I Y L G S N R A S G V P D R F S G S G S G T D F T L K I S R V E A E D V G V Y Y C M Q A L Q T
I L * * L S L H S P C P S P L E S R P P S P A G L V R A S C I V M D T T I W I G T C R S Q G S L H S S * S I W V L I G P P G S L T G S V A V D Q A Q I L H * K S A E W R L R M L G F I T A C K L Y K L
Y C D D S V S T L P A R H P W R A G L H L L Q V * S E P P A * * W I Q L F G L V P A E A R A V S T A P D L F G F * S G L R G P * Q V Q W Q W I R H R F Y T E N Q Q S G G * G C W G L L L H A S S T N
FR1-IMGT   1..78
  AA_IMGT AA 1 to 26
gatattgtgatgactcagtctccactctccctgcccgtcacccctggagagccggcctccatctcctgcaggtctagt D I V M T Q S P L S L P V T P G E P A S I S C R S S
I L * * L S L H S P C P S P L E S R P P S P A G L
Y C D D S V S T L P A R H P W R A G L H L L Q V *
1st-CYS   67..69
CDR1-IMGT   79..111
  AA_IMGT AA 27 to 38, AA 33 is missing
cagagcctcctgcatagtaatggatacaactat Q S L L H S N G Y N Y
R A S C I V M D T T
E P P A * * W I Q L
FR2-IMGT   112..162
  AA_IMGT AA 39 to 55
ttggattggtacctgcagaagccagggcagtctccacagctcctgatctat L D W Y L Q K P G Q S P Q L L I Y
W I G T C R S Q G S L H S S * S
G L V P A E A R A V S T A P D L
CONSERVED-TRP   118..120
CDR2-IMGT   163..171
  AA_IMGT AA 56 to 65, AA 58, 59, 60, 61, 62, 63 and 64 are missing
ttgggttct L G S
W V
G F
FR3-IMGT   172..279
  AA_IMGT AA 66 to 104, AA 73, 81, 82 are missing
aatcgggcctccggggtccctgacaggttcagtggcagtggatcaggcacagattttacactgaaaatcagcagagtggaggctgaggatgttggggtttattactgc N R A S G V P D R F S G S G S G T D F T L K I S R V E A E D V G V Y Y C
I G P P G S L T G S V A V D Q A Q I L H * K S A E W R L R M L G F I T
S G L R G P * Q V Q W Q W I R H R F Y T E N Q Q S G G * G C W G L L L
2nd-CYS   277..279
CDR3-IMGT   280..309
  AA_IMGT AA 105 to 117, AA 110, 111 and 112 are missing
atgcaagctctacaaactctcatgtgcagt M Q A L Q T L M C S
C K L Y K L S C A
A S S T N S H V Q
JUNCTION   277..312
  in_frame
tgcatgcaagctctacaaactctcatgtgcagtttt C M Q A L Q T L M C S F
A C K L Y K L S C A V
H A S S T N S H V Q F
3'V-REGION   277..298
tgcatgcaagctctacaaactc C M Q A L Q T
A C K L Y K L
H A S S T N
N-REGION   299
P-REGION   300..301
5'J-REGION   302..312
tgtgcagtttt C A V
V Q F
C S F
J-REGION   302..339
  IMGT_allele IGKJ2*04
  IMGT_gene IGKJ2
tgtgcagttttggccaggggaccaagctggagatcaaa C A V L A R G P S W R S
V Q F W P G D Q A G D Q
C S F G Q G T K L E I K
J-PHE   310..312
FR4-IMGT   310..339
  AA_IMGT AA 118 to 127
tttggccaggggaccaagctggagatcaaa F G Q G T K L E I K
L A R G P S W R S
W P G D Q A G D Q
C-REGION   340..>342
CL   340..>342

Literature references

[1] 1-342
Giachino C., Padovan E., Lanzavecchia A.
k+l+ dual receptor B cells are present in the human peripheral repertoire
Unpublished
[2] 1-342
Giachino C.
Submitted to the ENA/GenBank/DDBJ databases (07-NOV-1994)
Giachino C., Basel Institute for Immunology, Grenzacherstrasse 487, Basel, Switzerland, 4005
[3] 1-342
Giachino C., Padovan E., Lanzavecchia A.
kappa+lambda+ dual receptor B cells are present in the human peripheral repertoire
J. Exp. Med. 181(3):1245-1250 (1995)
DOI: 10.1084/jem.181.3.1245
PUBMED: 7869042

Database cross-references

Database Identifiers Relation
ENA Z46620

Sequence

Length : 342 BP
Composition : 78 A; 88 C; 91 G; 85 T; 0 other

     gatattgtga tgactcagtc tccactctcc ctgcccgtca cccctggaga gccggcctcc        60
     atctcctgca ggtctagtca gagcctcctg catagtaatg gatacaacta tttggattgg       120
     tacctgcaga agccagggca gtctccacag ctcctgatct atttgggttc taatcgggcc       180
     tccggggtcc ctgacaggtt cagtggcagt ggatcaggca cagattttac actgaaaatc       240
     agcagagtgg aggctgagga tgttggggtt tattactgca tgcaagctct acaaactctc       300
     atgtgcagtt ttggccaggg gaccaagctg gagatcaaac ga                          342
Created:
23/07/1995
Database development:
Denys CHAUME, Patrice DUROUX, Nelly JOUFFRE
Database scientific officer:
Marie-Paule Lefranc