IMGT/LIGM-DB logo
Database contains
247103 sequences
from 361 species

IMGT/LIGM-DB sequence

Version:
1.2.9
Database release:
LIGMDB_V12
Citing IMGT/LIGM-DB:
Giudicelli, V., Duroux P., Ginestoux C., Folch G., Jabado-Michaloud J., Chaume D., Lefranc M.-P., Nucleic Acids Res., 34:D781-D784 (2006). PMID:16381979 Abstract Full PDF

Views

Z70260 - Annotation

Sections
Accession number Z70260
Sequence version 1
Secondary accession numbers
IMGT annotation level by annotators
Definition H.sapiens mRNA for immunoglobulin kappa chain V-J region (patient 8).
Species Homo sapiens (human)
Taxonomy cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Primates; Haplorrhini; Simiiformes; Catarrhini; Hominoidea; Hominidae; Homininae; Homo; Homo sapiens
Sequence length 327
IMGT/LIGM-DB dates 30-APR-1996
  18-JAN-2013 (v. 6)

OBTENTION

Specificities
Disease related

IDENTIFICATION

Molecule type cDNA
Configuration type undefined, rearranged
Gene type variable, joining
Molecule entity type
Functionality type productive
Chain type IG-Light, IG-Light-Kappa-IGK
Structure type regular
Location type
Other keywords antigen receptor, Immunoglobulin superfamily (IgSF), immunoglobulin (IG), IMGT reference sequence

CLASSIFICATION

IMGT Groups IGKV
IMGT Subgroups IGKV3

DESCRIPTION

V-J-C-REGION   1..327
  cell_type lymphocyte
  db_xref PID:e229783
  dev_stage adult
  isolate patient 8 (RM)
  organism Homo sapiens
  tissue_type myeloma bone marrow
gaaattgtgttgacgcagtctccaggcaccctgtctttgtctccaggggaaagagccaccctcctctgcagggccagtcagagtgttagcagcaactacttagcctggtaccagcagaaacctggccagcctcccaggctcctcatctatagaacattcagcagggcccctggcatcccagacaggttcagtggcagtgggtctgggacagacttcactctcaccatcagcagactggagcctgaagattttgcggtgtatttctgtcagcagcatgttaccgaacagtgcacttttggccaggggaccaagctggagatcaaacgt E I V L T Q S P G T L S L S P G E R A T L L C R A S Q S V S S N Y L A W Y Q Q K P G Q P P R L L I Y R T F S R A P G I P D R F S G S G S G T D F T L T I S R L E P E D F A V Y F C Q Q H V T E Q C T F G Q G T K L E I K R
K L C * R S L Q A P C L C L Q G K E P P S S A G P V R V L A A T T * P G T S R N L A S L P G S S S I E H S A G P L A S Q T G S V A V G L G Q T S L S P S A D W S L K I L R C I S V S S M L P N S A L L A R G P S W R S N
N C V D A V S R H P V F V S R G K S H P P L Q G Q S E C * Q Q L L S L V P A E T W P A S Q A P H L * N I Q Q G P W H P R Q V Q W Q W V W D R L H S H H Q Q T G A * R F C G V F L S A A C Y R T V H F W P G D Q A G D Q T
V-J-REGION   1..324
gaaattgtgttgacgcagtctccaggcaccctgtctttgtctccaggggaaagagccaccctcctctgcagggccagtcagagtgttagcagcaactacttagcctggtaccagcagaaacctggccagcctcccaggctcctcatctatagaacattcagcagggcccctggcatcccagacaggttcagtggcagtgggtctgggacagacttcactctcaccatcagcagactggagcctgaagattttgcggtgtatttctgtcagcagcatgttaccgaacagtgcacttttggccaggggaccaagctggagatcaaa E I V L T Q S P G T L S L S P G E R A T L L C R A S Q S V S S N Y L A W Y Q Q K P G Q P P R L L I Y R T F S R A P G I P D R F S G S G S G T D F T L T I S R L E P E D F A V Y F C Q Q H V T E Q C T F G Q G T K L E I K
K L C * R S L Q A P C L C L Q G K E P P S S A G P V R V L A A T T * P G T S R N L A S L P G S S S I E H S A G P L A S Q T G S V A V G L G Q T S L S P S A D W S L K I L R C I S V S S M L P N S A L L A R G P S W R S
N C V D A V S R H P V F V S R G K S H P P L Q G Q S E C * Q Q L L S L V P A E T W P A S Q A P H L * N I Q Q G P W H P R Q V Q W Q W V W D R L H S H H Q Q T G A * R F C G V F L S A A C Y R T V H F W P G D Q A G D Q
V-REGION   1..287
  CDR_length [7.3.9]
  IMGT_gene IGKV3-20
gaaattgtgttgacgcagtctccaggcaccctgtctttgtctccaggggaaagagccaccctcctctgcagggccagtcagagtgttagcagcaactacttagcctggtaccagcagaaacctggccagcctcccaggctcctcatctatagaacattcagcagggcccctggcatcccagacaggttcagtggcagtgggtctgggacagacttcactctcaccatcagcagactggagcctgaagattttgcggtgtatttctgtcagcagcatgttaccgaaca E I V L T Q S P G T L S L S P G E R A T L L C R A S Q S V S S N Y L A W Y Q Q K P G Q P P R L L I Y R T F S R A P G I P D R F S G S G S G T D F T L T I S R L E P E D F A V Y F C Q Q H V T E
K L C * R S L Q A P C L C L Q G K E P P S S A G P V R V L A A T T * P G T S R N L A S L P G S S S I E H S A G P L A S Q T G S V A V G L G Q T S L S P S A D W S L K I L R C I S V S S M L P N
N C V D A V S R H P V F V S R G K S H P P L Q G Q S E C * Q Q L L S L V P A E T W P A S Q A P H L * N I Q Q G P W H P R Q V Q W Q W V W D R L H S H H Q Q T G A * R F C G V F L S A A C Y R T
FR1-IMGT   1..78
  AA_IMGT 1 to 26
gaaattgtgttgacgcagtctccaggcaccctgtctttgtctccaggggaaagagccaccctcctctgcagggccagt E I V L T Q S P G T L S L S P G E R A T L L C R A S
K L C * R S L Q A P C L C L Q G K E P P S S A G P
N C V D A V S R H P V F V S R G K S H P P L Q G Q
1st-CYS   67..69
CDR1-IMGT   79..99
  AA_IMGT AA 27 to 38, AA 31, 32, 33, 34 and 35 are missing
cagagtgttagcagcaactac Q S V S S N Y
R V L A A T
E C * Q Q L
FR2-IMGT   100..150
  AA_IMGT 39 to 55
ttagcctggtaccagcagaaacctggccagcctcccaggctcctcatctat L A W Y Q Q K P G Q P P R L L I Y
* P G T S R N L A S L P G S S S
S L V P A E T W P A S Q A P H L
CONSERVED-TRP   106..108
CDR2-IMGT   151..159
  AA_IMGT AA 56 to 65, AA 58, 59, 60, 61, 62, 63 and 64 are missing
agaacattc R T F
E H
N I
FR3-IMGT   160..267
  AA_IMGT 66 to 104,AA 73,81,82 missing
agcagggcccctggcatcccagacaggttcagtggcagtgggtctgggacagacttcactctcaccatcagcagactggagcctgaagattttgcggtgtatttctgt S R A P G I P D R F S G S G S G T D F T L T I S R L E P E D F A V Y F C
A G P L A S Q T G S V A V G L G Q T S L S P S A D W S L K I L R C I S
Q G P W H P R Q V Q W Q W V W D R L H S H H Q Q T G A * R F C G V F L
2nd-CYS   265..267
CDR3-IMGT   268..294
  AA_IMGT AA 105 to 117, AA 110, 111, 112 and 113 are missing
cagcagcatgttaccgaacagtgcact Q Q H V T E Q C T
S S M L P N S A
A A C Y R T V H
JUNCTION   265..297
tgtcagcagcatgttaccgaacagtgcactttt C Q Q H V T E Q C T F
V S S M L P N S A L
S A A C Y R T V H F
J-REGION   288..324
  IMGT_allele IGKJ2*02
  IMGT_gene IGKJ2
gtgcacttttggccaggggaccaagctggagatcaaa V H F W P G D Q A G D Q
C T F G Q G T K L E I K
A L L A R G P S W R S
J-PHE   295..297
C-REGION   325..327

Literature references

[1] 1-327
Sahota S.S., Leo R., Hamblin T.J., Stevenson F.K.
Myeloma VL gene sequences reveal somatic hypermutation with intraclonal homogeneity, and a role for VL in antigen selection
Unpublished
[2] 1-327
Sahota S.S.
Submitted to the ENA/GenBank/DDBJ databases (20-MAR-1996)
Surinder S Sahota, Molecular Immunology Group, Tenovus Research, Laboratory, Southampton University Hospitals, Tremona Road, Southampton, SO16 6YD, UNITED KINGDOM

Database cross-references

Database Identifiers Relation
ENA Z70260

Sequence

Length : 327 BP
Composition : 76 A; 93 C; 86 G; 72 T; 0 other

     gaaattgtgt tgacgcagtc tccaggcacc ctgtctttgt ctccagggga aagagccacc        60
     ctcctctgca gggccagtca gagtgttagc agcaactact tagcctggta ccagcagaaa       120
     cctggccagc ctcccaggct cctcatctat agaacattca gcagggcccc tggcatccca       180
     gacaggttca gtggcagtgg gtctgggaca gacttcactc tcaccatcag cagactggag       240
     cctgaagatt ttgcggtgta tttctgtcag cagcatgtta ccgaacagtg cacttttggc       300
     caggggacca agctggagat caaacgt                                           327
Created:
23/07/1995
Database development:
Denys CHAUME, Patrice DUROUX, Nelly JOUFFRE
Database scientific officer:
Marie-Paule Lefranc