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Mouse models

Mouse model for chronic lymphocytic leukemia (CLL)

IgH.TEµ CLL mouse model

The IgH.TEµ CLL mouse model is based on sporadic expression of the SV40 large T oncogene in mature B cells (SV40 large T insertion in opposite transcriptional orientation into the IGH locus D - J region) [1].

Because of the mixed sv129xC57BL/6 background of the IgH.TEµ CLL (F1 sv129xC57BL/6), IgMa/IgMb allotype expression is used to define CLL incidence by the accumulation of >70% IgMb+ B-cells in the peripheral blood of the mice. Two distinct unmutated CLL subsets are present in the IgH.TEµ mouse model. The IGHV11-2/IGHV14-126 expressing CLL developed independently of T cell help, and the non-IGHV11-2 CLL which is T cell dependent and displaying a specific transcriptional signature associated with non-stereotypic U-CLL in human [2].

[1] ter Brugge PJ, Ta VB, de Bruijn MJ, Keijzers G, Maas A, van Gent DC, Hendriks RW. A mouse model for chronic lymphocytic leukemia based on expression of the SV40 large T antigen. Blood (2009) 114:119-27. doi: 10.1182/blood-2009-01-198937

[2] Singh SP, de Bruijn MJW, de Almeida MP, Meijers RWJ, Nitschke L, Langerak AW, Pillai SY, Stadhouders R, Hendriks et al. Identification of Distinct Unmutated Chronic Lymphocytic Leukemia Subsets in Mice Based on Their T Cell Dependency. Front Immunol. 13 September 2018. doi: 10.3389/fimmu.2018.01996