IMGT notes:

(1)	The sequence described in the manuscript is different from the one submitted to the EMBL data library. Nucleotide a at position 261 in EMBL flat file (codon 11 in FR1-IMGT), nucleotide g in the manuscript.
(2)	No INIT-CODON in L-PART1, and non canonical V-HEPTAMER: cacagcg instead of cacagtg.
(3)	In frame STOP-CODON (positions 300-302) at codon 44 in FR2-IMGT.
(4)	Four nucleotides of the V-REGION of the V104A sequence from Givol et al. [5] were corrected by Blankestein et al. [30]. Nucleotides gc at positions 1096-1097 in EMBL flat file are replaced by nucleotides ca (g148>c, c149>a, A55>Q in the IMGT unique V-REGION numbering), nucleotide g at position 1111 is replaced by nucleotide a (g163>a, G60>S in CDR2-IMGT) and nucleotide c at position 1132 is replaced by g (c184>g, Q69>E in FR3-IMGT).
(5)	In frame STOP-CODON (positions 1031-1033) at codon 102 in FR3-IMGT, and non canonical V-HEPTAMER: cacagcg instead of cacagtg.
(6)	In frame STOP-CODON (positions 309-311) at codon 41 instead of the CONSERVED-TRP in FR2-IMGT.
(7)	In frame STOP-CODON (positions 319-321) at codon 44 in FR2-IMGT.
(8)	No INIT-CODON in L-PART1, and non canonical V-HEPTAMER: gacagtg instead of cacagtg.
(9)	In frame STOP-CODON (positions 62-64) at codon 1 in FR1-IMGT.
(10)	In frame STOP-CODON (positions 286-288) at codon 55 in FR2-IMGT.
(11)	Partial V-REGION: partial FR1-IMGT (AA 1 to 16 are absent).
(12)	Partial V-REGION: partial FR1-IMGT (AA 1 to 16 are absent) and partial FR3-IMGT (AA 100 to 104 are absent).
(13)	Partial V-REGION: partial FR1-IMGT (AA 1 to 16 are absent) and partial FR3-IMGT (AA 98 to 104 are absent). This sequence differs by 2 nucleotides from K02154 and is temporarily considered as an allele of this sequence, but these data need to be confirmed with a germline sequence.
(14)	Partial V-REGION: partial FR1-IMGT (AA 1 to 3 are absent).
(15)	In frame STOP-CODON (positions 530-532) at codon 90 in FR3-IMGT.
(16)	Partial V-REGION: partial FR1-IMGT (AA 1 to 3 are absent) and partial FR3-IMGT (AA 100 to 104 are absent).
(17)	No INIT-CODON in L-PART1.
(18)	Partial V-REGION: partial FR3-IMGT (AA 100 to 104 are absent).
(19)	Partial V-REGION: partial FR1-IMGT (AA 1 to 3 are absent) and partial FR3-IMGT (AA 81 to 104 are absent).
(20)	Partial V-REGION: partial FR1-IMGT (AA 1 to 11 are absent).
(21)	Sterile transcript.
(22)	Sterile transcript, partial V-REGION: partial FR1-IMGT (AA 1 to 3 are absent) and partial FR3-IMGT (AA 81 to 104 are absent).
(23)	In frame STOP-CODON (positions 300-302) at codon 44 in FR2-IMGT.
(24)	Partial V-REGION: partial FR1-IMGT (AA 1 to 10 are absent).
(25)	In frame STOP-CODON (positions 236-238) at codon 51 in FR2-IMGT, 1st-CYS is replaced by Arg, and INSERTION of 1 nucleotide (g at position 401) in the V-HEPTAMER.
(26)	Partial V-REGION: partial FR3-IMGT (AA 93 to 104 are absent), in frame STOP-CODON at codon 51 in FR2-IMGT. This sequence differs by 1 nucleotide from D13202 and is temporarily considered as an allele of this sequence, but these data need to be confirmed with a germline sequence.
(27)	Partial V-REGION: partial FR1-IMGT (AA 1 to 16 are absent).
(28)	Partial V-REGION: partial FR3-IMGT (AA 77 to 104 are absent), no CDR3-IMGT.
(29)	INSERTION of 1 nucleotide (c at position 684) in the V-HEPTAMER.
(30)	Non canonical V-HEPTAMER: cacattg instead of cacagtg.
(31)	No INIT-CODON in L-PART1.
(32)	In frame STOP-CODON (positions 375-377) at codon 44 in FR2-IMGT.
(33)	This sequence differs by 1 nucleotide from M34983 in the V-REGION and is temporarily considered as an allele of this sequence, but these data need to be confirmed with a germline sequence.
(34)	No INIT-CODON in the L-PART1, DELETION of 1 nucleotide (between positions 395/396) in FR2-IMGT and non canonical V-HEPTAMER: tacagtg instead of cacagtg.
(35)	Partial V-REGION: partial FR3-IMGT (AA 93 to 104 are absent), no CDR3-IMGT.. These sequences were isolated from liver genomic DNA. Most of these sequences are probably germline but some could be from B cells rearranged sequences. In order to assess the level of B lymphocyte contamination in liver, quantative PCR assay was performed. The level of rearranged templates in liver DNA is about one rearranged VH186.2-related gene for every 1000-4000 unrearranged VH186.2-related templates (Rothenfluh in Ph.D thesis) citation in [61]. Based on this observation, the authors consider that their sequences are germline.
(36)	DELETION of 1 nucleotide (between positions 787/788) in FR2-IMGT.
(37)	In frame STOP-CODON (positions 888-890) at codon 67 in FR3-IMGT.
(38)	In frame STOP-CODON (positions 851-853) at codon 54 in FR2-IMGT.
(39)	DELETION of 1 nucleotide (between positions 786/787) in FR1-IMGT.
(40)	CONSERVED-TRP is replaced by a STOP-CODON.
(41)	L26866 and J00535 are mentioned as identical in Weiller et al. [68], the nucleotide t missing between positions 63/64 in L26866 EMBL flat file seems to be a typing error.
(42)	This sequence differs by 1 nucleotide from L26858 in the V-REGION and is temporarily considered as an allele of this sequence, but these data need to be confirmed with a germline sequence.
(43)	This sequence differs by 1 nucleotide from L33936 in the V-REGION and is temporarily considered as an allele of this sequence, but these data need to be confirmed with a germline sequence.
(44)	DELETION of 1 nucleotide (between positions 838/839) in FR2-IMGT.
(45)	In frame STOP-CODON (positions 957-959) at codon 89 in FR3-IMGT, no CDR3-IMGT..
(46)	In frame STOP-CODON (positions 793-795) at codon 27 in CDR1-IMGT.
(47)	This sequence differs by 1 nucleotide from L26863 in the V-REGION and is temporarily considered as an allele of this sequence, but these data need to be confirmed with a germline sequence.
(48)	J00502 (761 bp) and M36690 (509 bp) are from the same clone but differ by one nucleotide (g at position 441 in J00502, t at position 188 in M36690) at codon 13 in FR1-IMGT. Comparison with other sequences indicates that the J00502 has the correct sequence.
(49)	This sequence differs by 1 nucleotide from X03399 in the V-REGION.
(50)	The nucleotide c at position 271 in the EMBL flat file is a typing error because this nucleotide is a g in the manuscript (c271>g in the IMGT unique V-REGION numbering.)
(51)	The nucleotide c at position 271 in the EMBL flat file is a typing error because this nucleotide is a g in the manuscript (c271>g in the IMGT unique V-REGION numbering.) This sequence differs by 1 nucleotide from K02791 in the V-REGION and is temporarily considered as an allele of this sequence, but these data need to be confirmed with a germline sequence.
(52)	Partial V-REGION: partial FR3-IMGT (AA 92 to 104 are absent), no CDR3-IMGT.
(53)	Partial V-REGION: partial FR3-IMGT (AA 102 to 104 are absent), no CDR3-IMGT.
(54)	Partial V-REGION: partial FR2-IMGT (AA 1 to 54 are absent).
(55)	Partial V-REGION: partial FR1-IMGT (AA 1 to 7 are absent), partial FR3-IMGT (AA 101 to 104 are absent), no CDR3-IMGT.
(56)	JUNCTION is out_of_ frame .
(57)	These sequences were isolated from liver genomic DNA by PCR using a sense FR1 primer and a FR3 antisense primer. They are probably germline but this needs to be confirmed with a germline sequence.
(58)	This sequence differs by 4 nucleotides from K02890 in the V-REGION and is temporarily considered as an allele of this sequence, but these data need to be confirmed with a germline sequence.
(59)	This sequence differs by 4 nucleotides from X00163 in the V-REGION and is temporarily considered as an allele of this sequence, but these data need to be confirmed with a germline sequence.
(60)	This sequence differs by 1 nucleotide from X03400 in the V-REGION and is temporarily considered as an allele of this sequence, but these data need to be confirmed with a germline sequence.
(61)	This sequence differs by 5 nucleotides from X67409 in the V-REGION and is temporarily considered as an allele of this sequence, these data need to be confirmed with a germline sequence.
(62)	DELETION of 1 nucleotide (between positions 248/249) in the L-PART2 and in frame STOP-CODON (positions 428-430) at codon 67 in the FR3-IMGT.
(63)	Partial V-REGION: partial FR1-IMGT (AA 1 to 12 are absent).
(64)	Partial V-REGION: partial FR1-IMGT (AA 1 to 23 are absent).
(65)	Partial V-REGION: partial FR1-IMGT (AA 1 to 10 are absent).
(66)	Partial V-REGION: partial FR1-IMGT (AA 1 to 24 are absent).
(67)	Partial V-REGION: partial CDR2-IMGT and no FR3-IMGT.
(68)	Partial V-REGION: partial FR1-IMGT (AA 3 to 5 are absent ), partial FR3-IMGT (AA 67 to 104 are absent).
(69)	V00758 is also part of J00538 (positions 443-856) which is a sequence constructed from data of 4 different sources. See note (90).
(70)	In frame STOP-CODON (positions 151-153) at codon 52 in FR2-IMGT, STOP-CODON at codon 98 in FR3-IMGT, INSERTION of 4 nucleotides caca (position 281-284) in FR3-IMGT.
(71)	The nucleotides c at position 151 and g at position 292 in the J00524 EMBL flat file are typing errors (c151>g and g292>c). The sequences J00500 and J00524 are identical.
(72)	This sequence is mentioned as identical to the V3 sequence from Crews et al. [4], in [34], the nucleotide a at position 773 in the M16724 EMBL flat file (instead of g, in J00504 [4]) may represent a typing error.
(73)	The nucleotides t at position 140, t at position 144 and a at position 145 in the J00526 EMBL flat file seem to be typing errors (t140>c, t144>a, a145>g).
(74)	DELETION of 1 nucleotide (between positions 440/441) in FR2-IMGT and DELETION of 3 nucleotides (between positions 487/488) in CDR2-IMGT.
(75)	In frame STOP-CODON at codon 14 in FR1-IMGT, in frame STOP-CODON at codon 106 in CDR3-IMGT, and non canonical V-HEPTAMER: ctcattg instead of cacagtg.
(76)	Partial V-REGION: partial FR3-IMGT (AA 103 to 104 are absent), no CDR3-IMGT.
(77)	Partial V-REGION: partial FR3-IMGT (AA 103 to 106 are absent), in frame STOP-CODON (positions 146-148) at codon 52 in FR2-IMGT.
(78)	Partial V-REGION: partial FR3-IMGT (AA 104 is absent), in frame STOP-CODON at codon 103 in FR3-IMGT.
(79)	Partial V-REGION: partial FR3-IMGT (AA 102 to 104 are absent), no CDR3-IMGT..
(80)	Partial V-REGION: partial CDR3-IMGT (AA 105 to 106 are absent). This sequence differs by 1 nucleotide from Z15022 and is temporarily considered as an allele of this sequence, but these data need to be confirmed with a germline sequence.
(81)	Partial V-REGION: partial CDR3-IMGT (AA 105 to 106 are absent).
(82)	Partial V-REGION: partial FR1-IMGT (AA 1 to 3 are absent), partial CDR3-IMGT (AA 105 to 106 are absent).
(83)	This sequence differs by 2 nucleotides from AF064442 in the V-REGION and is temporarily considered as an allele of this sequence, but these data need to be confirmed with a germline sequence.
(84)	This sequence differs by 3 nucleotides from AF064443 in the V-REGION and is temporarily considered as an allele of this sequence, but these data need to be confirmed with a germline sequence.
(85)	DELETION of 1 nucleotide in the FR1-IMGT leading to a frameshift.
(86)	This sequence differs by 1 nucleotide from the putative germline sequence from Chao et al. [50].
(87)	This sequence is identical to the putative germline sequence from Chao et al. [50].
(88)	INSERTION of 1 bp in FR1-IMGT and INSERTION of 1 bp in FR3-IMGT leading to frameshifts.
(89)	JUNCTION is out_of_ frame .
(90)	J00538 is a sequence constructed from data of 4 different sources. Positions 443-856 correspond to the germline sequence from V00758. Other parts of the sequence are derived from rearranged clones and need to be confirmed by genomic germline data.
(91)	M34984 differs from M34983 by two additional nucleotides (nucleotides ga) at the end of the CDR3-IMGT.
(92)	Partial V-REGION: partial FR1-IMGT (AA 1 to 2 are absent), no CDR3-IMGT.
(93)	No INIT-CODON in L-PART1.

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Created: 23/03/2001
Last updated: Friday, 22-Sep-2023 18:19:15 CEST
Author: Christèle Martinez-Jean