Probe: pH60 clone (TRGJ probe) [1] [2].
The pH60 clone cross-hybridizes to the TRGJ1 and TRGJ2 genes.

TRGJ gene name RFLP alleles Enzymes Fragments (kb) [3] (1) Polymorphism type (2) Frequencies NCBI genome
French
[4]		 Lebanese
[4]		 Tunisian
[4]		 Black African
[4]		 Europeans
[5]
Caucasoid[4,6]
TRGJ1 *A1 HindIII 2.1 RS         0.99 [6] 6968
*A2 3.7 0.01 [6]
*B1 EcoRI 1.5   1.00 [4]
TRGJ2 *A1 HindIII 2.1 RS 0.65 0.52 0.55 0.29 0.67 [5] 6969 [4][5]
*A2 5 0.35 0.48 0.45 0.71 0.33 [5]
*B1 EcoRI 1.5 RS 0.95 0.95 1.00 1.00  
*B2 3.2 0.05 0.05 0 0  

IMGT notes:

(1) The probe used in the original paper [3] was M13H60 [1] ; the pH60 probe, shorter than M13H60 and without internal EcoRI site in the insert, is prefered for the EcoRI RFLP studies. Both probes cross-hybridize to the TRGJ1 and TRGJ2 genes.
(2) RS, polymorphic Restriction Site.

References:

[1] Lefranc, M.-P. and Rabbitts, T.H., Nature, 316, 464-466 (1985).
[2] Lefranc, M.-P. et al., Nature, 319, 420-422 (1986).
[3] Lefranc, M.-P. et al., Proc. Natl. Acad. Sci. USA, 83, 9596-9600 (1986).
[4] Buresi, C. et al., Immunogenetics, 29, 161-172 (1989).
[5] Sakkas, L.I. et al., J. Rheumatol, 17, 1487-1490 (1990).
[6] Weill, M. et al., Hum. Genet., 88, 355 (1992).

Created: 12/05/1999
Last updated: Friday, 22-Sep-2023 18:22:37 CEST
Authors: Violaine Moreau and Marie-Paule Lefranc