Part 1 - Phage availability and characteristics

Schematic maps of the phages are available at the foot of the JCRB phage pages.

Phage name Cell line (1) Insert size (kb) Gene content Insert extension ATCC number HGMP number JCRB number Accession number (3)
lambdaepsilonalphaTOU [1] (2,3) 19.5 IGHE and IGHA2 From just the 5' end of the IGHE BamHI (2.8 kb) fragment to approximately 4.3 kb downstream of the IGHA2 CH3 exon. 65694/65695 1168 HG287 J00221/M35287/M35291
lambdaEZZgamma3 [2] EZZ
[4,5] (3)		 19 IGHG3 65690/65691 1166 HG285 X03604/ X03604
X04646
lambdaepsilon10EZZ [3] EZZ
[4,5](3)		 14.4 IGHE From about 7.2 kb upstream of the IGHE BamHI (2.8 kb) fragment to about 4.4 kb downstream of this fragment. 65692/65693 1167 HG286

Part 2 - Phage restriction fragments and localisation of the insert restriction sites

Phage name Sizes of the phage restriction digest fragments (4) Localisation of the insert restriction sites
(kb from the 5' insert end except if mentioned) (4)
BamHI EcoRI HindIII PvuI SacI SmaI XbaI XhoI BamHI EcoRI HindIII
lambdaepsilonalphaTOU [1] 21.0
16.0
9.2
2.8
0.6		 21.0
13.7
9.2
5.5		 21.0
9.5(X2)
8.0
1.7		 21.0
9.2
5.4
4.6
2.6
2.3
1.0(X2)		 21.0
14.0
9.2
5.2		 2.8
18.7		 13.7 9.5
17.5
lambdaepsilon10EZZ [2] 21.0
9.2
7.2
4.4
2.8		 21.0
14.4
9.2
(6)		 21.0
9.2
8.8
3.9
0.90
0.70		 21.0
12.0
8.2
5.8		 21.0
9.2
3.6
2.6
2.3
1.8
1.1
1.0
0.65
0.58
0.52		 21.0
5.8
3.7
3.5
1.25
0.90
0.84
0.76		 21.0
11.0
9.2
3.8		 21.0
14.4
9.2
(6)		 7.2
10		 (6) 3.9
4.8
5.5
lambdaEZZgamma3 [3] 21.0
10.0
9.2
4.0
2.1		 23.0
17.5
9.2
(5)		 28.0
9.2
7.2
4.5		 21.0
12.0
2.9
2.1
1.7		 21.0
11.5
9.2
2.4
1.5
1.0
0.78		 21.0
8.8
5.8
3.1
2.7
1.2
1.1
0.74
0.45		 21.0
19.0
9.2		 21.0
11.5
9.2
7.7		 2.1
12.1
16.1		 17.5 0.5
5.0
12.3
IMGT notes:
(1) Human cell lines from which DNA was extracted for the construction of the lambda phage genomic libraries.
(2) The phage genomic library was constructed from TOUII-5 [4,5] DNA extracted from total peripheral white blood cells.
(3) EZZ and TOU [4] are TOUII-4 and TOU-5, respectively, in the family tree [5]. EZZ (TOUII-4) EBV transformed cell line is available at ECACC (n° 93093003).
(4) Sizes in kb are from experimental data and are approximate. Sizes of the vector arms are 21 kb and 9.2 kb.
(5) One site at 1.6 kb from the 3' insert end [3].
(6) No site in the insert.
References:
[1] Lefranc, M.-P. and Rabbitts, T.H., Nucl. Acids Res., 12, 1303-1311 (1984).
[2] Huck, S. et al., Nucl. Acids Res., 14, 1779-1789 (1986).
[3] Keyeux, G. and al., Genomics, 5, 431-441 (1989).
[4] Lefranc, M.-P. et al., Nature, 300, 760-762 (1982).
[5] Lefranc, M.-P. et al., Mol. Biol. Med., 1, 207-217 (1983).