Part 1 - Probe and clone availability

TRGC
gene name		 Probe Clone name ATCC number HGMP number JCRB number Accession number
TRGC1, TRGC2 TRGC-ex1 pC1BH0.8 [1,2] 61220/61221 1190 HG202 M14996
mC1BH0.8 [1] 59610/59611 HG171
TRGC-ex2 pEX2TBP [4] 61232/61233 1033 HG198 M25318
TRGC-ex3 pC1R0.9 [1,3] 61222/61223 866 HG206 M14998
mC1R0.9 [1] 59612/59613 HG172

Part 2 - Probe and clone characteristics

Clone name Gene content Insert size (bp) Restriction sites Vector Phage (1) Cell line (2) Comments
pC1BH0.8 [1,2] 3' part of TRGC1-exon1 and its 3' UTR 800 BamHI - HindIII pUC lambdaD19 [1] D-PLL Cross-hybridizes with TRGC2 exon1
mC1BH0.8 [1] M13
pEX2TBP [4] TRGC2 exon 2T 136 BamHI - PstI pUC lambdaR12 [6] RPMI 8402 Cross-hybridizes with exons 2 (EX2T, 2R and 2) of TRGC2 and TRGC1 exon 2.
pC1R0.9 [1,3] TRGC1-exon3 840 EcoRI pUC lambdaD19 [1] D-PLL Cross-hybridizes with TRGC2 exon3
mC1R0.9 [1] M13mp9

Part 3 - Main probe uses

In this table, are shown for each gene, the most appropriate probes to use for TRG gene rearrangements, for RFLP, and for TRG gene expression analyses. Probes are designated by their clone names. Several probes are available in both pUC and M13 (names starting with "p" or "m", respectively). Note that clones in pUC are easier to handle and to grow than clones in M13. Click on the clone names for more information on TRG gene rearrangements and on RFLP data.

TRGC
gene name		 For TRGC gene rearrangements For RFLP For TRGC gene expression
TRGC1, TRGCC2 pEX2TBP, mC1R0.9, pC1R0.9, mC1BH0.8, pC1BH0.8 [4,7,8,9] mC1R0.9, pC1R0.9, mC1BH0.8, pC1BH0.8
IMGT notes:
(1) Phages from which have been isolated the clones used as probes.
(2) Human cell lines from which DNA was extracted for construction of the phage genomic libraries. They are either alpha-beta T cell or B cell lines. Note that all the data on the TRG genes have been obtained from cells which do not express the TcR gamma-delta receptor. When cell lines expressing the TcR gamma chain have been identified [Brenner, M.B et al., Nature, 322, 145-149 (1986)], the whole of the data on the TRG gene organisation and rearrangement obtained in alpha-beta T cells and B cells [1,2,3,5],has been shown to be valid for gamma-delta T cells.
References:
[1] Lefranc, M.-P. and Rabbitts, T.H., Nature, 316, 464-466 (1985).
[2] Lefranc, M.-P. et al., Nature, 319, 420-422 (1986).
[3] Lefranc, M.-P. et al., Proc. Natl. Acad. Sci. USA, 83, 9596-9600 (1986).
[4] Buresi, C. et al., Immunogenetics, 29, 161-172 (1989).
[5] Lefranc, M.-P. et al., Cell, 45, 237-246 (1986).
[6] Foster, A. et al., EMBO J., 6, 1945-1950 (1987).
[7] Pelicci, P.G. et al., Science, 237, 1051-1055 (1987).
[8] Li, Y. et al., J. Immunol., 140, 1300-1303 (1988).
[9] Ghanem, N. et al., Hum. Genet., 86, 450-456 (1991).