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Database contains
1864 primers

IMGT/PRIMER-DB Primer card: IPP800001

PRIMER CATALOGUE
IMGT/PRIMER-DB Primer accession number IPP800001
IMGT/PRIMER-DB entry date 21/11/2003
IMGT/PRIMER-DB update 24/11/2003
IMGT/PRIMER-DB Primer definition Homo sapiens IGKV2D-29*01 Sense primer Sense
Primer alias name AF103
Bibliographic references
(PubMed)		 PMID: 11261927
Catalogue comments IGKV2D-29 gene specific
PRIMER CHARACTERISTICS
IMGT/PRIMER-DB Primer sequence 27 pb; 8 A; 2 C; 6 G; 11 T; 0 other

5' TATTTAAGCTTGGGTTCATTTAAAGAG 3'
IMGT/PRIMER-DB Sequence length (number of nucleotides) 27
IMGT/PRIMER-DB Primer orientation Sense
IMGT/LIGM-DB reference sequence
Accession number		 M31952
IMGT/LIGM-DB reference sequence
Primer position (start-end)		 584-610
Primer Localization 
...GGAGATGAGGGAGGAGGAGGGGGTGGGAAGCTGAGC   420
TCTGGCGGCCCCACTGATTCCCGTGTTTATTCTAACCATGTGTTAAAGGAATATGGCCTA   480
TGCTCCAGGGAGAGGAATTCATATTTTGCCCTGATGATGATTTGAAAACTCCTAAAAGCA   540
GTGCTCTGAATAATATCTTGAGAAATGAAAGAACTCTTGTGCCTATTTAATAAAGGGTTC   600
ATTTAAAGAGTTTGTTTTTATGATATGAATACAAATTTGTAAAAATAAAAGATTAGCCAT   660
AAATCAATACCATAAGGCAAATCTCAAAAGTTGTTCATTATGCTTTCACATAACCTTGCA   720
CTTCTCTCTCATAATTTCAGGATCCAGTGCAGATATTGTGATGACCCAGACTCCACTCTC   780
TCTGTCCGTCACCCCTGGACAGCCGGCCTCCA...   1241
Characteristics comments IGKV2D-29*01 Allele chosen as a representative of the IGKV2D-29 Gene
IMGT/PRIMER-DB Set IPS000122
PRIMER CLASSIFICATION
IMGT/LIGM-DB reference sequence
Classification		 Species Homo sapiens
IMGT group IGKV
IMGT subgroup IGKV2
IMGT gene name IGKV2D-29
IMGT allele name IGKV2D-29*01
Classification comments and specificity AF103 and AF68 for IGKV2D-29 allele determination by PCR and restriction enzyme mapping
PRIMER DESCRIPTION
IMGT/PRIMER-DB Primer labels
PRIMER 1..27
V-GENE 1..27
V-INTRON 1..27
Differences between
the IMGT/LIGM-DB reference sequence
and the primer sequence		 Modified: T8 is replaced by G8 in primer sequence
Modified: A9 is replaced by C9 in primer sequence
Modified: A10 is replaced by T10 in primer sequence
Modified: A11 is replaced by T11 in primer sequence
Restriction enzyme name  
Restriction site position  
Purification methods  
Keywords  
Description comments  
Created:
22/02/2002
Last updated:
21/11/2006
Developped and maintained:
Julien Bertrand (LIGM, Montpellier, France), Géraldine Folch (EUROGENTEC) and François Ehrenmann (LIGM, Montpellier, France).
Scientific responsibles:
Marc Lemaître (EUROGENTEC, Belgium) and Marie-Paule Lefranc (LIGM, France) Marie-Paule.Lefranc@igh.cnrs.fr