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Unproductive transcript from MOPC21 cell line

Frequent amplification of the unproductive rearranged kappa variable IGKV-J gene from MOPC21, is observed during PCR of V genes from hybridomas.

References and accession numbers for the sequence of the unproductive rearranged IGKV-J from MOPC21:

This unproductive rearranged IGKV-J transcript is found in the MOPC21-derived fusion partners (mouse myeloma cells) such as NS-1, P3-X63-Ag8.653, SP2/0 and P3U1 (P3X63Ag8U.1).


H+ and L+ indicate the expression of the productive Ig heavy and light transcripts in MOPC1 and P3-X63-Ag8. H- and L- indicate the absence of expression of the endogenous productive transcripts in the MOPC1 derived fusion partners. However the unproductive rearranged kappa transcript is still found.

In NS0, a non-IG secreting H-L- cell line, derived from NS-1, a frameshift mutation in the L-KAPPA mRNA is responsible of the loss of the kappa chain expression. Hybridomas made with NS0 contain this unproductive transcript.

Different approaches have been proposed to eliminate the unproductive transcript:

For more information on PCR and cloning of murine V genes, see also:
More on MOPC21 and P3:
IMGT References:
[1] Potter, M., Apella, E. and Geisser, S., J. Mol. Biol., 14, 361-372 (1965). PMID: 4160338
[2] Horibata, K. and Harris, A.W., Exp. Cell Res., 60, 61-77 (1970). PMID: 5439579
[3] Svasti, J. and Milstein, C., Biochem. J., 126, 837-850 (1972). PMID: 5073237
[4] Svasti, J. and Milstein, C., Biochem. J., 128, 427-444 (1972). PMID: 4638343
[5] Hamlyn P.H. et al. Nucleic Acids Res. 9, 4485-4494 (1981). PMID: 6170937
See also: