Rearrangements (RFLP): Human TRG

A unique probe pH60 can detect all the TRG rearrangements whatever the TRGJ genes involved in the rearrangements (Huck, S. and Lefranc, M.-P., FEBS Lett., 224, 291-296 (1988)).

Click here to see Part 1 - Assignments of the TRG rearrangements to given TRGV genes (joined to TRGJ1 or TRGJ2)


Part 2 - Assignments of the TRG rearrangements to given TRGJ genes by hybridization of KpnI digests

Rearrangements of the TRGV genes to the additional TRGJ genes, JP, JP1 and JP2, can be identified by hybridization of the KpnI digests to the probe pH60 [4]. Sizes of the rearranged bands are shown in kilobases. The TRGV1 subgroup genes known to rearrange (the functional TRGV2, TRGV3, TRGV4, TRGV5 and TRGV8, and the pseudogene TRGV7) [2] and the unique TRGV3 subgroup gene (TRGV10) give bands of the same size when rearranged to a given TRGJ gene.

Reference for this table: Foster, A. et al., EMBO J., 6, 1945-1950 (1987).

Probe: pH60 clone (TRGJ probe)[1] [3].

TRGJ
gene		 TRGV subgroup
1 2 3 4
J1, J2 1.8 7.5(7.8) 1.8 6.0
JP1 8.5 14.5 8.5 12.7
JP1 5.9 12.0 5.9 10.0
JP2 4.7 10.7 4.7 9.0

References:
[1] Lefranc, M.-P. and Rabbitts, T.H., Nature, 316, 464-466 (1985).
[2] Lefranc, M.-P. et al., Cell, 45, 237-246 (1986).
[3] Lefranc, M.-P. et al., Nature, 319, 420-422 (1986).
[4] Huck, S. and Lefranc, M.-P., FEBS Lett., 224, 291-296 (1988).

Created: 12/05/1999
Authors: Violaine Moreau and Marie-Paule Lefranc