Rearrangements (RFLP): Human TRG

A unique probe pH60 can detect all the TRG rearrangements whatever the TRGJ genes involved in the rearrangements (Huck, S. and Lefranc, M.-P., FEBS Lett., 224, 291-296 (1988)).

Click here to see Part 2 - Assignments of the TRG rearrangements to given J genes by hybridization of KpnI digests


Part 1 - Assignments of the TRG rearrangements to given TRGV genes (joined to TRGJ1 or TRGJ2)

Since the TRGJ1 and TRGJ2 genes are highly homologous [3], it is possible, with the TRGJ1 probe, pH60 [1] [3], to detect the TRGV rearrangements to TRGJ1 and TRGJ2. Moreover, the sizes of the rearranged restriction fragments when pH60 is hybridized to BamHI, HindIII and EcoRI digests, allow allocation to a given TRGV of the rearragements observed in whole thymus, T cell lines or T cell leukemias [4]. This table shows the rearranged restriction fragments corresponding to the nine TRGV genes known to rearrange [2] [4].

Reference for this table: Foster, A. et al., EMBO J., 6, 1945-1950 (1987).

Probe: pH60 clone (TRGJ probe)[1] [3].

TRG subgroup TRG
gene name		 BamHI HindIII EcoRI
TRGV1 V2 11.7 4.3 0.9
V3 16 3.7 5.4(+V2)
V4 21 4.3 0.9
V5 25 3.6 2.2
V7 35 2.9 3.1
V8 40 2.9 4.2
TRGV2 V9 15.5 4.0 2.4
TRGV3 V10 19 5.1 0.65
TRGV4 V11 12 5.6 9.5

References:
[1] Lefranc, M.-P. and Rabbitts, T.H., Nature, 316, 464-466 (1985).
[2] Lefranc, M.-P. et al., Cell, 45, 237-246 (1986).
[3] Lefranc, M.-P. et al., Nature, 319, 420-422 (1986).
[4] Foster, A. et al., EMBO J., 6, 1945-1950 (1987).
[5] Huck, S. and Lefranc, M.-P., FEBS Lett., 224, 291-296 (1988).

Created: 12/05/1999
Authors: Violaine Moreau and Marie-Paule Lefranc