IGL group | IGL gene name | Probe | Clone name | ATCC number | HGMP number | JCRB number | Accession number |
---|---|---|---|---|---|---|---|
IGLJ | J2 | IGLJ2 and IGLJ3 | pJlambda2 [1] | 59616/59617 | 865 | HG174 |
Clone name | Gene content | Insert size (bp) | Restriction sites | Vector | Phage (1) | Cell line | Comments |
---|---|---|---|---|---|---|---|
pJlambda2 [1] | IGLJ2 | 2000 | BglII - EcoRI | pUC 9 | Chr22lambda5 [2] (2) | cross-hybridizes only with the IGLJ2 and IGLJ3 containing fragments and therefore only detects the polymorphic EcoRI fragments. |
(1) | Phages from which have been isolated the clones used as probes. |
(2) | Available, subcloned in pUC, under clone name pclambda22 at HGMP-RC probe 1215. |
[1] | Ghanem, N. et al., Exp. Clin. Immunogenet., 5, 186-195 (1988). |
[2] | Rabbitts, T.H. et al., Mol. Biol. Med., 1, 11-19 (1983). |