Part 1 - Probe and clone availability

TRGV subgroup TRGV
gene name		 Probe Clone name ATCC number HGMP number JCRB number Accession number
1 TRGV1, TRGV2, TRGV3, TRGV4, TRGV5, TRGV5P, TRGV6, TRGV7, TRGV8 VgammaI pV3S [1] 59592/59593 1188 HG162 M13430
VgammaI-J (1) S12SR [2] 59590/59591 845 HG161 M13824/X03437
2 TRGV9 VgammaII pV9PH [3] 59596/59597 847 HG164 X07205
VgammaII-J (2) K20PR [1] 59594/59595 846 HG163 M13823/X03436
3 TRGV10 VgammaIII-5' pV10PR [3] 59598/59599 848 HG165 X05503
VgammaIII-3' pV10RB [4] 61218/61219 855 HG205 X07206
mV10RB [4] 59600/59601 HG166
4 TRGV11 VgammaIV-5' pV11SPRS [4] 59604/59605 849 HG168 X07207
mV11SPRS [4] 59602/59603 HG167
A TRGVA TRGVA-5' p5A6 [1] 59606/59607 850 HG169
TRGVA-3' pVA0.6H [3] 59608/59609 851 HG170 X07208
B TRGVB TRGVB pVB0.5KH [4] 61224/61225 852 HG200 X07209
mVB0.5KH [4] 59614/59615 HG173

Part 2 - Probe and clone characteristics

Clone name Gene content Insert size (bp) Restriction sites Vector Phage (3) Cell line (4) Comments
pV3S [1] TRGV3 1200 SacI pUC lambdaSH4 [1] B cell line SH Cross-hybridizes with all TRGV1 subgroup genes (V1, V2, V3, V4, V5, V5P, V6, V7, V8)
S12SR [2] TRGV3-J2 1200 SacI - EcoRI pUC12 lambdaS12 [1,2] SUPT1
pV9PH [3] TRGV9 700 PstI - HindIII pUC lambdaSHV7 [3] B cell lymphoblastoïd
K20PR [1] TRGV9-J1 1000 PstI - EcoRI pUC lambdaK20 [2] Karpas 1010 A 410 bp PstI-AccI fragment isolated from K20PR contains TRGV9 (alone) and can be used as a VgammaII probe. [3,7]
pV10PR [3] 5' part of TRGV10 600 PstI - EcoRI pUC lambdaR12 [3] RPMI 8402
pV10RB [4] 3' part of TRGV10 400 EcoRI - BamHI pUC lambdaJM15 [4] JM To get the insert, digest with EcoRI and HindIII (BamHI not reconstituted)
mV10RB [4] M13 mp19
pV11SPRS [4] 5' part of TRGV11 270 SphI - RsaI pUC19 lambdaJM15 [4] JM To get the insert, digest with EcoRI and HindIII
mV11SPRS [4] M13mp19
p5A6 [1] 5' part of TRGVA and its 5' UTR 2550 SacI - HindIII pUC19 lambdaA6 [1] AT5B1 SacI is a vector site
pVA0.6H [3] 3' part of TRGVA and its 3' UTR 600 HindIII pUC lambdaA6 [1] AT5B1
pVB0.5KH [4] 3' part of TRGVB and its 3' UTR 500 KpnI - HindIII pUC lambdaJM15 [4] JM
mVB0.5KH [4] M13

Part 3 - Main probe uses

In this table, are shown for each gene, the most appropriate probes to use for TRG gene rearrangements, for RFLP, and for TRG gene expression analyses. Probes are designated by their clone names. Several probes are available in both pUC and M13 (names starting with "p" or "m", respectively). Note that clones in pUC are easier to handle and to grow than clones in M13. Click on the clone names for more information on TRG gene rearrangements and on RFLP data.

TRGV subgroup TRGV
gene name		 For TRGV gene rearrangements For RFLP For TRGV gene expression
1 TRGV1, TRGV2, TRGV3, TRGV4, TRGV5, TRGV5P, TRGV6, TRGV7, TRGV8 pH60 [7] pV3S [8,9,12] pV3S
2 TRGV9 pH60 [7] pV9PH [13] pV9PH
3 TRGV10 pH60 [7] pV10PR
4 TRGV11 pH60 [7] pV11SPRS, mV11SPRS [10,11] pV11SPRS
A TRGVA p5A6, pVA0.6H
B TRGVB pVB0.5KH, mVB0.5KH
IMGT notes:
(1) The clone S12SR contains a rearranged V3-J2 gene.
(2) The clone K20PR contains a rearranged V9-J1 gene.
(3) Phages from which have been isolated the clones used as probes.
(4) Human cell lines from which DNA was extracted for construction of the phage genomic libraries. They are either alpha-beta T cell or B cell lines. Note that all the data on the TRG genes have been obtained from cells which do not express the TcR gamma-delta receptor. When cell lines expressing the TcR gamma chain have been identified [Brenner, M.B et al., Nature, 322, 145-149 (1986)], the whole of the data on the TRG gene organisation and rearrangement obtained in alpha-beta T cells and B cells [1,2,5,6],has been shown to be valid for gamma-delta T cells.
References:
[1] Lefranc, M.-P. et al., Cell, 45, 237-246 (1986).
[2] Lefranc, M.-P. et al., Nature, 319, 420-422 (1986).
[3] Foster, A. et al., EMBO J., 6, 1945-1950 (1987).
[4] Huck, S. et al., EMBO J., 7, 719-726 (1988).
[5] Lefranc, M.-P. and Rabbitts, T.H., Nature, 316, 464-466 (1985).
[6] Lefranc, M.-P. et al., Proc. Natl. Acad. Sci. USA, 83, 9596-9600 (1986).
[7] Huck, S. et Lefranc, M.-P., FEBS lett., 224, 291-296 (1987).
[8] Font, M.-P. et al., J. Exp. Med., 168, 1383-1394 (1988).
[9] Ghanem, N. et al., Immunogenetics, 30, 350-360 (1989).
[10] Ghanem, N. et al., Nucleic Acids Res., 17, 1270 (1989).
[11] Ghanem, N. et al., Nucleic Acids Res., 17, 1271 (1989).
[12] Ghanem, N. et al., Hum. Genet., 86, 450-456 (1991).
[13] Martinez-Naves, E. et al., Nucleic Acids Res., 19, 5806 (1991).