Part 1 - Probe and clone availability

TRGJ
gene name		 Probe Clone name ATCC number HGMP number JCRB number Accession number
TRGJP1, TRGJP2 TRGJP1 p16HS [1,4] 61234/61235 854 HG199 X08084
TRGJP TRGJP p58R [1,2] 61216/61217 853 HG201 M12950
m58R [1] 59588/59589 HG160
TRGJ1, TRGJ2 TRGJ1 pH60 [1,3] 59584/59585 96 HG158 M12960/X03434
M13H60 [1,3] 59586/59587 HG159

Part 2 - Probe and clone characteristics

Clone name Gene content Insert size (bp) Restriction sites Vector Phage (1) Cell line (2) Comments
p16HS [1,4] TRGJP1 1000 HindIII - SacI pUC lambdaRgamma [1] Raji Cross-hybridizes with JP2
p58R [1,2] 3' part of TRGJP 1300 EcoRI pUC lambdaRgamma [1] Raji
m58R [1] M13
pH60 [1,3] TRGJ1 700 HindIII - EcoRI pUC9 lambdaRgamma [1] (3) Raji This probe can detect all the TRGV rearrangements to the five TRGJ segments when it hybridizes to KpnI digested DNAs. [6] Click here for sizes of the rearrranged fragments.

Part 3 - Main probe uses

In this table, are shown for each gene, the most appropriate probes to use for TRG gene rearrangements, for RFLP, and for TRG gene expression analyses. Probes are designated by their clone names. Several probes are available in both pUC and M13 (names starting with "p" or "m", respectively). Note that clones in pUC are easier to handle and to grow than clones in M13. Click on the clone names for more information on TRG gene rearrangements and on RFLP data.

TRGJ
gene name		 For TRGJ gene rearrangements For RFLP For TRGJ gene expression
TRGJP1, TRGJP2 pH60 [4] p16HS
TRGJP pH60 [4] p58R, m58R
TRGJ1, TRGJ2 pH60 [4] pH60 [7,8]
IMGT notes:
(1) Phages from which have been isolated the clones used as probes.
(2) Human cell lines from which DNA was extracted for construction of the phage genomic libraries. They are either alpha-beta T cell or B cell lines. Note that all the data on the TRG genes have been obtained from cells which do not express the TcR gamma-delta receptor. When cell lines expressing the TcR gamma chain have been identified [Brenner, M.B et al., Nature, 322, 145-149 (1986)], the whole of the data on the TRG gene organisation and rearrangement obtained in alpha-beta T cells and B cells [1,2,3,5], has been shown to be valid for gamma-delta T cells.
(3) pH60 has been isolated from the M13H60 [1] clone (2.1 kb insert in M13mp18 vector), itself isolated from the lambdaRgamma phage [1].
References:
[1] Lefranc, M.-P. and Rabbitts, T.H., Nature, 316, 464-466 (1985).
[2] Lefranc, M.-P. et al., Cell, 45, 237-246 (1986).
[3] Lefranc, M.-P. et al., Nature, 319, 420-422 (1986).
[4] Huck, S. and Lefranc, M.-P., FEBS lett., 224, 291-296 (1987).
[5] Lefranc, M.-P. et al., Proc. Natl. Acad. Sci. USA, 83, 9596-9600 (1986).
[6] Huck, S. et al., EMBO J., 7, 719-726 (1988).
[2] Buresi, C. et al., Immunogenetics, 29, 161-172 (1989).
[3] Sakkas, L.I. et al., J. Rheumatol, 17, 1487-1490 (1990).