The IGHC protein display numbering is according to the IMGT unique numbering for C-DOMAIN and C-LIKE-DOMAIN.
N (Asn, asparagine) of potential N-glycosylation sites (NXS/T, where X is different from P), (N-linked glycosylation) is shown is green (site is underlined in CHS and in pages edited before 14/10/2009).
The hinge exon(s) are shown at the end of the protein display.
_________________________________________________________________________________________________________________________________________________________________________________ IGHC genes _________________________________________________________________________________________________________________________________________________________________________________ A AB B BC C CD D DE E EF F FG G CHS --------------> ----------> ------> -------> -----------> -------> ----------> 1 10 15 16 20 23 30 36 39 41 45 77 84 85 89 96 97 104 110 118 121 130 140 150 7654321|........|....|123|...|...... ...|.....| |.....|1234567|......|12345677654321|..........|12|....... .....|....... ..|.........|.........|.........| CH1 AJ421266,IGHG1 c (A)STKAPSVYPLTARCGDTP...GSTVAFGCLVW GYIP..EPVT VTWNSGALSS....GIHTFPSVLMSS......GLYSLSSLVTLPASSS...SGKNFIC NVAHPA..SSTKV DKRV AJ131945,IGHG2 (1) (A)STKAPSVYPLTARSGDTP...GSTVAFGCLVW GYIP..EPVT VTWNSGAVSS....GIHTFPSVLMSL......GLYSLSSLVTLPTSTS...TGKTFIS NVAHPA..SSTKV DKSV CH2 AJ421266,IGHG1 c (A)PELPGGPSVFVFPPKPKDVLSI.SGRPEVTCVVV DVGQEDPEVN FNWYIDGVEVR...TANTKPKEEQFN......STYRVVSVLTIQHQDW..LTGKEFKC KVNNKA..LPAPI ERTISKAK AJ131945,IGHG2 (A)PELLGGPSVFIFPPKPKDVLSI.SGRPEVTCVVV DVGQEDPEVS FNWYIDGVEVR...TANTKPKEEQFN......STYRVVSVLTIQHQDW..LTGKELKC KVNNKA..LPAPI ERTISKAK Z48947 ,IGHG3 c (A)PELPGGPSVFVFPPKPKDVLSI.SGRPEVTCVVV DVGKEDPEVN FNWYIDGVEVR...TANTKPKEEQFN......STYRVVSVLTIQHQDW..LTGKEFKC KVNNKA..LPAPI ERTISKPK CH3 AJ421266,IGHG1 c (G)QTREPQVYTLAPHREELA...KDTVNVTCLVK GFYP..PDIN VEWQRNGQPESEG.AYATTLPQQDND......GTYFLYSKLSVGKNTW..QQGETFTC VVMHEA.LHNHST QKSITQSS...GK AJ131945,IGHG2 (G)QTREPQVYTLAPHREELA...KDTVSITCLVI GFYP..ADIN VEWQRNGRPESEG.AYATTLPQLDND......GTYFLYSKLSVGKNTW..QQGETFTC VVMHEA.LHNHST QKSITQSS...GK Z48947 ,IGHG3 c (G)QTREPQVYTLAPHREELA...KDTVSVTCLVK GFYP..PDIN VEWQRNRQPESEG.AYATTLPQLDND......GTYFLYSKLSVGKNTW..QRGETFTC VVMHEA.LHNHYT QKSITQSS...GK M1 M2 AJ131945,IGHG2 (E)PLLEEESCAEAQSGELDGLWTTISIFITLFLLSVCYSATVTLFK VKWIFSSVVELKRTIVPDYRNMIGQGS CONNECTING-REGION | TRANSMEMBRANE-REGION | CYTOPLASMIC-REGION Hinge AJ421266,IGHG1 c (E)LEISEPQPQPGCTCPKCP AJ131945,IGHG2 (E)PKIPQPQPKPQPQPQPQPKPQPKPEPECTCPKCP Z48947 ,IGHG3 c (G)TNEVCKCPKCP
c: cDNA sequence
(1) | Note that this CH1 domain is not expressed in the camel IGH gamma 2 chain. Indeed, owing to a splicing defect of the CH1 (non canonical DONOR-SPLICE) the V-D-J-REGION is spliced to the hinge exon (H), which leads to the absence of the CH1 domain in the camel IGH gamma 2 chain. The conserved Cys 104 of the CH1 (IMGT numbering for C-DOMAIN) is replaced by Ser. |
See: Characteristics of the camelidae (camel, llama) antibody synthesis