Colors are according to IMGT Color menu for sequence alignments and Color menu for CDR-IMGT

• Amino acids are shown in the one-letter abbreviation.
• Hydrophobic amino acids (hydropathy index with positive value) and Tryptophan (W) found at a given position in more than 50% of analysed IG and TR sequences are shown in blue.
• All Proline (P) are shown in yellow.
• Missing positions in IMGT Colliers de Perles based on 3D structures may be due to incomplete experimental data.
• Arrows indicate the direction of the beta sheets and their different designations in 3D structures. This information has to be used carefully if not supported by experimental data.
• The CDR-IMGT are limited by amino acids shown in squares, which belong to the neighbouring FR-IMGT. The CDR3-IMGT extend from position 105 to position 117.
• CDR-IMGT regions are colored as follows: CDR1-IMGT (blue), CDR2-IMGT (green), CDR3-IMGT (greenblue).
• C at position 23 (1st-CYS), W at position 41 (CONSERVED-TRP), letter of the conserved hydrophobic amino acid at position 89, and C at position 104 (2nd-CYS) are in red. If an amino acid change occurs at these crucial positions, the letter will remain red.
• Hatched circles or squares correspond to missing positions according to the IMGT unique numbering for V-DOMAIN (Lefranc et al. 2003 PMID: 12477501 ).

IMGT notes:
The proteolytic cleavage site between the leader peptide and the V-REGION is putative and the assignment of amino acid 1 to one or the other region is uncertain.